Using Acetate and Formate as the Substrates for <i>Geobacter sulfurreducens</i> Exoelectrogenesis Resulted in Different Half-saturation Potentials

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  • PENG Luo
    School of Resources & Environment, Southwest University Biofunctional Catalysts Research Team, RIKEN Center for Sustainable Resource Science
  • ZHANG Xiao-Ting
    School of Resources & Environment, Southwest University
  • KAWAICHI Satoshi
    Biofunctional Catalysts Research Team, RIKEN Center for Sustainable Resource Science
  • XIE De-Ti
    School of Resources & Environment, Southwest University
  • LI Zhen-Lun
    School of Resources & Environment, Southwest University

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  • Using Acetate and Formate as the Substrates for Geobacter sulfurreducens Exoelectrogenesis Resulted in Different Half-saturation Potentials

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Abstract

In bioelectrochemical systems where the oxidative current is mediated by microorganisms, it remains unexplored as to whether low-potential substrates (e.g. formate) enable the anode to work at lower potentials. Due to implications to relevant engineering and natural systems, this study evaluated such possibility and underlying causes. The investigation compared voltammograms of the model exoelectrogen (to exclude the interfering factors in undefined cultures) Geobacter sulfurreducens grown with acetate and formate. G. sulfurreducens had an EM (half-saturation potential) of −0.138 ± 0.004 V vs. SHE when consuming acetate; an EM of −0.160 ± 0.002 V when utilizing formate. Such variation usually requires alternation in electrode reductase expressed by bacteria, according to the existing Nernst-Monod model with a single species of electron conduit. For both acetate- and formate-grown biofilm, non-catalytic voltammetries found multiple redox couples with distinct formal potentials. No clear evidence could support a hypothesis that the bacteria synthesized any different electron conduits when the substrate was changed. Significant changes in the relative abundance of high-potential and low-potential electrocatalytically active conduits were not observed as well. However, low-potential conduits showed elevated electrocatalytic activities in the formate-grown biofilm, which might induce the shift in apparent EM.

Journal

  • Electrochemistry

    Electrochemistry 83 (8), 600-604, 2015

    The Electrochemical Society of Japan

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