Molecular Weight Dependence of Column Efficiency in Gel Permeation Chromatography and Reverse Flow Technique

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Other Title
  • 〔61〕 GPCにおけるカラム性能の分子量依存性とReversal法について

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Description

The relation between column efficiency and molecular weight of a polymer sample in gel permeation chromatography was studied.<BR>Usually, a column efficiency in the number of theoretical plates per foot is measured from a chromatogram of organic solvents, such as acetone and benzene, This method has a disadvantage that it is unsuitable for estimating undesirable broadening of chromatograms of a high molecular weight sample.<BR>From our experiments with reverse flow technique, the following approximate relation of the height equivalent to the theoretical plate, H, and molecular weight of the sample, M, was obtained.<BR>logH=A+BlogM<BR>In this relation, the information about undesirable broadening of chromatograms in the high molecular weight region in GPC was given.<BR>The reverse flow technique is a useful one in GPC to obtain a column efficiency at high molecular weight region. The application of this technique is also described in this report.

Journal

  • Kobunshi Kagaku

    Kobunshi Kagaku 27 (304), 498-504, 1970

    The Society of Polymer Science, Japan

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Details 詳細情報について

  • CRID
    1390282681495889280
  • NII Article ID
    130004032867
  • DOI
    10.1295/koron1944.27.498
  • ISSN
    18848079
    00232556
  • Text Lang
    ja
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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