Direct Observations of High Resolution 1H NMR in Liquid Phase for Peptides bound to Bicelles

DOI
  • KAWAGUCHI Ken
    Analytical Sciences Department, Toray Research Center, Inc. Department of Biotechnology, Tokyo University of Agriculture and Technology
  • KIMURA Kazuo
    Analytical Sciences Department, Toray Research Center, Inc.
  • ASAKURA Tetsuo
    Department of Biotechnology, Tokyo University of Agriculture and Technology

Bibliographic Information

Other Title
  • バイセルに結合したペプチドの高分解能｀１´Ｈ　ＮＭＲ観測

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Description

Direct observations of high resolution 1H NMR in liquid phase for Valinomycin bound to bicelles as a model of membrane bound peptides were endeavored using a high resolution magic angle spinning (HR-MAS) probe. The bicelles were composed of two kinds of lipids, dimyristoyl phosphatidylcholine (DMPC) and 3- [ (3-cholamidopropyl) dimethylanunonio] -2-hydroxypropane sulfonic acid (CHAPSO). The equivalent amounts of these lipids were mixed. Using both a HR-MAS probe and bicelles, we observed high resolution 1H NMR spectra of the peptide directly and successfully. Amide protons of valinomycin bound to bicelles were observed even in deuterium oxide solution and after sonication. Such results suggest that valinomycin forms intra molecular hydrogen bonds and is bound to bicelles. Assignments of the amide protons of valinomycin bound to bicelles were performed from the multiplicity and NOESY spectrum using a HR-MAS probe.

Journal

  • KOBUNSHI RONBUNSHU

    KOBUNSHI RONBUNSHU 60 (4), 199-202, 2003

    The Society of Polymer Science, Japan

Keywords

Details 詳細情報について

  • CRID
    1390282681498292608
  • NII Article ID
    130004035601
  • DOI
    10.1295/koron.60.199
  • ISSN
    18815685
    03862186
  • Text Lang
    ja
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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