Electron spectroscopy for chemical analysis studies on polypeptide surfaces.

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  • KUGO Kohei
    Department of Applied Chemistry, Faculty of Science, Konan University
  • KITAURA Tatsuro
    Department of Applied Chemistry, Faculty of Science, Konan University
  • NISHINO Jun
    Department of Applied Chemistry, Faculty of Science, Konan University

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Other Title
  • ポリペプチド表面のＥＳＣＡ分析
  • ポリペプチド ヒョウメン ノ ESCA ブンセキ

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Abstract

The outer-most surface characteristic of poly (γ-benzyl L-glutamate) (PBLG) and poly (ε-N-benzyloxycarbonyl L-lysinc) (PBCL) were investigated by electron spectroscopy for chemical analysis (ESCA). These homopolypeptides exist in the α-helicai conformation in the solid state. The ESCA measurements showed the ratios (O/C) of atomic concentrations of both polypeptides are in accord with the bulk values for grazing angles from 45°C to 60°C. As the result of the evaluation of effective sampling depth estimated from the electron mean free path, we obtained d=16.3Å to 19.9Å for PBLG and d= 16.6Å to 20.4Å for PBCL in the same grazing angle range. The diameters of the cross-section, a, of PBLG and PBCL helical rods have been reported as a=15.0Å and 18.0Å, respectively. Agreement of d- and a-values for PBLG and PBCL seems to be satisfactory. These results suggest that the depth profile of polypcptide surfaces existing in the α-helical conformation is specific and the effective sampling depths of both PBLG and PBCL surfaces are in accord with the diameters of the cross-section of their helical rods over the same range of grazing angles.

Journal

  • KOBUNSHI RONBUNSHU

    KOBUNSHI RONBUNSHU 45 (5), 441-447, 1988

    The Society of Polymer Science, Japan

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