Selective Cell Capture and Release Using Antibody-Immobilized Polymer-Grafted Surface

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  • KIMURA Tsuyoshi
    Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University
  • NAKAMURA Naoko
    College of Systems Engineering and Science, Shibaura Institute of Technology
  • HASHIMOTO Yoshihide
    Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University
  • SAKAGUCHI Shimon
    Immunology Frontier Research Center, Osaka University
  • KIMURA Shunsaku
    Graduate School of Engineering, Kyoto University
  • KISHIDA Akio
    Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University

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Other Title
  • 解離型分子を介した抗体固定化グラフト表面を用いた選択的な細胞捕獲・回収による細胞分離
  • カイリガタ ブンシ オ カイシタ コウタイ コテイカ グラフト ヒョウメン オ モチイタ センタクテキ ナ サイボウ ホカク ・ カイシュウ ニ ヨル サイボウ ブンリ

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Abstract

Cell separation is a powerful tool in life science research and clinical use. Various methods for cell separation have been developed. In this paper, we describe two cell separation methods using surface immobilizing antibodies via dissociation molecules. As one method, acrylic acid was grafted on the surface in order to inhibit non-specific cell adhesion and the antibody was immobilized via desthiobiotin-avidin interaction. Cells were selectively captured through antibody-antigen interaction and released by addition of biotin-modified polymer due to the exchange reaction of desthiobiotin and biotin. For the second method, the surface was modified by grafting poly(N-isopropylacrylamide-co-arylic acid) and the antibody was immobilized via double strand DNA. Cells were adhered on the surface at 37°C and detached by 4°C incubation with remaining cells that interacted with the surface via antibody-antigen interaction. The adhered cells were released by DNase treatment. These results suggest that cells can be selectively captured and collected by using the surface that immobilizes an antibody via dissociation molecules.

Journal

  • KOBUNSHI RONBUNSHU

    KOBUNSHI RONBUNSHU 75 (2), 155-163, 2018

    The Society of Polymer Science, Japan

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