書誌事項
- タイトル別名
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- Effects of epidermal growth factor(EGF) on invasive and metastatic ability of tumor cells. Part 2: Mechanisms of malignant phenotype acquisition by long-term EGF treatment.
- Part 2: Mechanisms of malignant phenotype acquisition by long-term EGF treatment
- 第2報, EGF長期間処理による悪性形質獲得機序の検討
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We have previously reported that epidermal growth factor (EGF) enhanced the invasive and metastatic abilities of regressor ER-1 cells and that ER-1 cells might acquire stable malignant phenotypes after long-term EGF treatment. In this study, we studied the mechanisms of the malignant phenotype acquisition by long-term EGF treatment.<BR>EGF enhanced the chemotactic response of ER-1 cells toward culture supernatant conditioned with rat lung endothelial cells in a dose-dependent fashion and also enhanced invasiveness into the reconstituted basement membrane (MatrigelTM) in a similar manner. Furthermore, when ER-1 cells were cultured in the presence of EGF for a month (1M EGF ER-1), they acquired stable invasiveness. In EGF binding assay using 125I-EGF, the numbers of EGF receptors were similar for ER-1 cells and 1 M EGF ER-1 cells, which expressed both highand low-affinity receptors. RT-PCR analysis demonstrated that mRNA expression of EGF receptors was similar for both ER-1 cells and 1M EGF ER-1 cells. Drug-resistance tests using c-SST-2 cells (parent cells of ER-1) revealed that colonies resistant to 6-thioguanine and ouabain appeared after long-term EGF treatment (one month).<BR>These results suggest that long-term EGF treatment may affect the genomic alteration of ER-1 cells and that these may acquire stable malignant phenotypes
収録刊行物
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- 日本口腔外科学会雑誌
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日本口腔外科学会雑誌 42 (3), 256-263, 1996
社団法人 日本口腔外科学会
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詳細情報 詳細情報について
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- CRID
- 1390282681503712768
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- NII論文ID
- 10011656978
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- NII書誌ID
- AN00189163
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- ISSN
- 21861579
- 00215163
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
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- 使用不可