細胞膜内におけるべん毛モータの立体構造

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  • Three-dimensional structure of bacterial flagellar motor in the cytoplasmic membrane.
  • サイボウ マク ナイ ニ オケル ベンモウ モータ ノ リッタイ コウゾウ

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The three-dimensional molecular architecture of Salmonella flagellar motor in the bacterial membrane was examined in situ, by quick-freeze deep-etch replica electron microscopy. The MS ring complex, the FliF gene product, which was over-expressed in E. coli appeared as a drilled bead sitting on a flat disk, projecting toward the periplasm. The cytoplasmic side of the intact flagella consisted of a large bell-shaped structure, which was identified to C ring. In its center, we found a new rod-like structure with a central hole, which we denominated as C rod. This structure may operate as an apparatus to selectively transport the flagellar proteins across the membrane.

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  • 生物物理

    生物物理 37 (2), 63-67, 1997

    一般社団法人 日本生物物理学会

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