<i>In situ</i> hybridization 法による壊死性口内炎組織中の<i>Aspergillus</i> の検出—血液疾患患者を対象とした予備的検討
書誌事項
- タイトル別名
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- Identification of <i>Aspergillus</i> species in oral tissue samples of patients with hematologic malignancies by <i>in situ</i> hybridization: A preliminary report
- 二次出版 In situ hybridization法による壊死性口内炎組織中のAspergillusの検出--血液疾患患者を対象とした予備的検討
- 2ジ シュッパン In situ hybridizationホウ ニ ヨル エシセイ コウナイエン ソシキ チュウ ノ Aspergillus ノ ケンシュツ ケツエキ シッカン カンジャ オ タイショウ ト シタ ヨビテキ ケントウ
- Identification of Aspergillus species in oral tissue samples of patients with hematologic malignancies by in situ hybridization: A preliminary report
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説明
Purpose: In the present study, we analyzed the usefulness of a DNA probe directed against the alkaline proteinase (ALP) gene of Aspergillus fumigatus to diagnose invasive oral aspergillosis using the in situ hybridization (ISH) technique in patients with hematologic malignancies.<br> Patients and Methods: The ALP probe was tested on tissue specimens from 16 patients with hematological malignancies who had invasive orofacial fungal infections and positive cultures: Aspergillus species in 13 patients as well as Exophiala dermatitis, Trichoderma longibrachiatum, and Candida albicans in 1 patient each. In situ hybridization with the ALP probe was done by using formalin-fixed, paraffin-embedded tissue samples.<br> Results: The ALP probe showed a strong reaction with specimens from all 13 patients who had culture-proven aspergillosis specimens. On the other hand, the ALP probe showed no cross-reactivity with three non-Aspergillus fungi.<br> Conclusions: These findings indicate that ISH using an ALP probe may increase the accuracy of diagnosing invasive oral aspergillosis in patients with hematologic malignancies and facilitate the provision of adequate antifungal treatment.
収録刊行物
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- 日本口腔外科学会雑誌
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日本口腔外科学会雑誌 55 (10), 482-489, 2009
社団法人 日本口腔外科学会
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詳細情報 詳細情報について
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- CRID
- 1390282681507740416
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- NII論文ID
- 10027091103
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- NII書誌ID
- AN00189163
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- ISSN
- 21861579
- 00215163
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- NDL書誌ID
- 10471481
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
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- 使用不可
