Effect of RNAi-induced inhibition of ets-1 gene expression on invasion properties of oral squamous carcinoma cells-Functional evaluation of ets-1 gene-
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- AKANE Mitsunori
- Second Department of Oral and Maxillofacial Surgery, Osaka Dental University
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- OHNISHI Yuichi
- Second Department of Oral and Maxillofacial Surgery, Osaka Dental University
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- KAKUDO Kenji
- Second Department of Oral and Maxillofacial Surgery, Osaka Dental University
Bibliographic Information
- Other Title
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- 口腔扁平上皮癌細胞の浸潤能に及ぼすRNAiを用いたets‐1遺伝子発現抑制の影響―ets‐1遺伝子の機能評価―
- コウクウ ヘンペイ ジョウヒガン サイボウ ノ シンジュンノウ ニ オヨボス RNAi オ モチイタ ets 1 イデンシ ハツゲン ヨクセイ ノ エイキョウ ets 1 イデンシ ノ キノウ ヒョウカ
- Functional evaluation of<I>ets</I>-1 gene
- <I>ets</I>-1遺伝子の機能評価
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Abstract
Metastasis in invasive carcinoma is closely associated with the production of extracellular matrix catabolic enzymes, such as matrix metalloproteinases (MMPs). It is known thatetsproto-oncogenes exist in transcriptional regulation refions of the MMP gene and enhance or inhibit MMP transcription. In this study, we restrained theets-1 gene expression of an oral squamous carcinoma cell line by means of RNA interference (RNAi) and examined the change in invasion ability from a molecular biological perspective. An oral squamous carcinoma cell line (HSC-3) was transfected with short interfering RNA (siRNA) ofets-1. The invasive ability of the transfected cells was determined by a matrigel invasion assay, and MMP-9 production was measured by gelatin zymography. These data were compared with those of normal cultured cells. Matrigel invasion assay demonstrated that the transfected cells had a lower invasive ability than HSC-3, and gelatin zymography demonstrated that the MMP-9 production in these cells had decreased. These results suggested that transfection of siRNAets-1 would inhibit tumor invasion through down-regulation of MMP-9.
Journal
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- Japanese Journal of Oral and Maxillofacial Surgery
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Japanese Journal of Oral and Maxillofacial Surgery 53 (1), 2-7, 2007
Japanese Society of Oral and Maxillofacial Surgeons
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Details 詳細情報について
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- CRID
- 1390282681508583168
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- NII Article ID
- 10018858475
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- NII Book ID
- AN00189163
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- ISSN
- 21861579
- 00215163
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- NDL BIB ID
- 8659025
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed