遺伝子組み換えヒト骨形成因子によるラット下腿筋肉内骨誘導におけるニコチンの影響

  • 園部 純也
    市立宇和島病院歯科口腔外科
  • 海原 真治
    京都桂病院歯科口腔外科
  • 大久保 康則
    京都大学大学院医学研究科感覚運動系外科学講座口腔外科学分野
  • 河井 まりこ
    岡山大学大学院医歯学総合研究科生体機能再生.再建学講座口腔形態学分野
  • 楠本 健司
    関西医科大学形成外科学講座
  • 別所 和久
    京都大学大学院医学研究科感覚運動系外科学講座口腔外科学分野

書誌事項

タイトル別名
  • Effect of nicotine on osteoinduction by recombinant human bone morphogenetic protein-2
  • イデンシ クミ カエ ヒト コツケイセイ インシ ニ ヨル ラット カタイ キンニク ナイコツ ユウドウ ニ オケル ニコチン ノ エイキョウ

この論文をさがす

抄録

We evaluated the effect of nicotine on osteoinduction by recombinant human bone morphogenetic protein-2 (rhBMP-2). Five micrograms of rhBMP-2 was implanted into the calf muscle of 10-week-old Wistar rats. For 14 days after implantation, nicotine was continuously administered subcutaneously in a dose of 0.1 (group L), 1.0 (group M), or 10.0 (group H) mg/kg/day. In the control group (group C), only physiological saline was administered. On day 21 after implantation, soft radiographs were obtained, and histological and biochemical analyses were performed. The results suggested that a high dose of nicotine pre ents bone formation.<BR>In addition, we investigated the effect of nicotine on osteoinduction. For 14 days after rhBMP-2 implantation, nicotine was continuously administered in the dose used in group M. The control group was given only physiological saline. On days 3, 7, 14, and 21 after implantation, soft radiographs were obtained after the injection of contrast media, and histological and biochemical analyses were performed. In the radiographic study with contrast media, there were fewer small capillaries in the rhBMP-2-implanted muscle in the nicotine group than in the control group. Histologically, nicotine reduced new bone and cartilage formation and decreased the number of capillaries. On quantitative biochemical analysis, indices of bone formation in the nicotine group were significantly lower than those in control. These results suggest that the prevention of bone formation by nicotine may be caused by a reduced blood supply to rhBMP-2-implanted muscle.

収録刊行物

参考文献 (11)*注記

もっと見る

関連プロジェクト

もっと見る

詳細情報 詳細情報について

問題の指摘

ページトップへ