Irregular Folding of Human Genome DNA in the Cells
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- MAESHIMA Kazuhiro
- Structural Biology Center, National Institute of Genetics Department of Genetics, School of Life Science, Sokendai
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- JOTI Yasumasa
- JASRI
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- NISHINO Yoshinori
- Research Institute for Electronic Science, Hokkaido University
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- TAKATA Hideaki
- Structural Biology Center, National Institute of Genetics Graduate School of Engineering Osaka University
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- KAMADA Fukumi
- Structural Biology Center, National Institute of Genetics
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- HIHARA Saera
- Structural Biology Center, National Institute of Genetics Department of Genetics, School of Life Science, Sokendai
Bibliographic Information
- Other Title
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- ヒトゲノムDNAの不規則で柔軟な収納原理
- ヒトゲノム DNA ノ フキソク デ ジュウナン ナ シュウノウ ゲンリ
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Abstract
How is 2-m-long genomic DNA organized into a mitotic chromosome or nucleus? The nucleosome fiber has long been assumed to be folded into a 30-nm chromatin fiber, and further helically folded larger fiber. However, when we observed frozen hydrated human mitotic cells using cryoelectron microscopy (cryo-EM), no higher-order structures including 30-nm chromatin fibers were found. To further investigate the bulk structure of mitotic chromosomes, we performed small-angle X-ray scattering (SAXS) at SPring-8. No structural feature larger than 11 nm was detected, even on a chromosome-diameter scale (~1 μm). We also found a similar scattering pattern in interphase nuclei of HeLa cells in the range up to ~275 nm. Our findings suggest a common structural feature in interphase and mitotic chromatins: compact and irregular folding of nucleosome fibers occurs without a 30-nm chromatin structure.<br>
Journal
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- Seibutsu Butsuri
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Seibutsu Butsuri 53 (1), 004-010, 2013
The Biophysical Society of Japan General Incorporated Association
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Keywords
Details 詳細情報について
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- CRID
- 1390282681510962944
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- NII Article ID
- 10031139018
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- NII Book ID
- AN00129693
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- COI
- 1:CAS:528:DC%2BC3sXisVWntr0%3D
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- ISSN
- 13474219
- 05824052
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- NDL BIB ID
- 024274438
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed