書誌事項
- タイトル別名
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- A Time-Resolved Detection Method of Protein-Protein Interaction and Its Applications
- ジカン ブンカイ タンパクシツカン ソウゴ サヨウ ケンシュツホウ ト ソノ オウヨウ
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抄録
A new type of biosensor is presented for detecting protein-protein interaction in solution phase in time-domain. A fast time resolution was achieved by the diffusion measurement with the transient grating method. This technique allows us to study the inter-protein interaction of short-lived intermediate species. This biosensor possesses many unique merits compared with the traditional biosensors, such as the surface plasmon resonance biosensor, and the merits were discussed. We applied this technique to the detection of various intermolecular interactions of photosensor proteins; such as a reaction intermediate of photoactive yellow protein (PYP) and phototropins. Transient photo-induced association and dissociation of intermediates were detected.<br>
収録刊行物
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- 生物物理
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生物物理 47 (4), 235-240, 2007
一般社団法人 日本生物物理学会
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詳細情報 詳細情報について
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- CRID
- 1390282681511151616
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- NII論文ID
- 110006369067
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- NII書誌ID
- AN00129693
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- ISSN
- 13474219
- 05824052
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- NDL書誌ID
- 8900588
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可