Semi-intact Cell System and Cell Resealing Technique for Image-based Analysis of Intracellular Events: A Powerful Cellular Tool for Single Cell Biology

DOI Web Site Web Site 8 References Open Access
  • MURATA Masayuki
    Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo
  • NOGUCHI Yoshiyuki
    Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo
  • HORIUCHI Yuta
    Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo
  • HORII Sakuya
    Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo
  • KANO Fumi
    Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo

Bibliographic Information

Other Title
  • セミインタクト細胞リシール法を用いた細胞内イベントの可視化解析―「一細胞生物学」の細胞側ツールとして―
  • セミインタクト サイボウ リシールホウ オ モチイタ サイボウ ナイ イベント ノ カシカ カイセキ : 「 イチ サイボウ セイブツガク 」 ノ サイボウガワ ツール ト シテ

Search this article

Description

Semi-intact cells are cells whose plasma membrane is permeabilized with toxins by creating pores. Semi-intact cells allow for the exchange of the original cytosolic proteins with an exogenously-made cytosol prepared from cells of varying states, such as cells at different stages of the cell cycle, differentiation, or cells in a diseased state. Recently we found that the pores in semi-intact cells can be resealed by calcium ions. Here, we introduce a basic protocol for preparing a “disease-model cell” using the resealing technique of semi-intact cells, in which the cytosolic conditions of a diabetic liver was replicated, and demonstrate the disturbances in endocytosis and signal transduction in the diabetic liver-model cells.

Journal

  • Seibutsu Butsuri

    Seibutsu Butsuri 54 (4), 206-209, 2014

    The Biophysical Society of Japan General Incorporated Association

References(8)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top