Catalytic Mechanism of Lysozyme Based on the Structures of Invertebrate-type Lysozyme and Chicken-type Lysozyme

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  • ABE Yoshito
    Laboratory of Protein Structure, Function and Design, Graduate School of Pharmaceutical Sciences, Kyushu University

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  • リゾチームの触媒機構：無脊椎動物型とニワトリ型の比較から
  • リゾチーム ノ ショクバイ キコウ : ムセキツイ ドウブツガタ ト ニワトリガタ ノ ヒカク カラ

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Abstract

<p>Based on the structure of invertebrate-type lyzozyme, Venerupis philippinarum lysozyme (VpL), we designed Asn46Asp/Asp52Ser or Asn46Glu/Asp52Ser hen egg white lysozyme (HEWL) mutants. Both lysozyme mutants possessed lysozyme acitivity like VpL and HEWL. Although Asn46Glu/Asp52Ser mutant formed a glycosyl adduct in the reaction with the N-acetylglucosamine oligomer, however, the turnover rate of estimated from the glycosyl formation and decomposition rates was only 20% of the observed hydrolysis rate of the substrate. From these results, we discussed the catalytic mechanism of lysozymes.</p>

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  • Seibutsu Butsuri

    Seibutsu Butsuri 57 (3), 140-143, 2017

    The Biophysical Society of Japan General Incorporated Association

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