Effect of £-Poly-l-lysine on a Glucose Sensor Based on Glucose Oxidase and Ferricyanide Ion
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- UEMATSU Kohei
- Department of Bioscience, Fukui Prefectural University
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- UENO Takaaki
- Department of Bioscience, Fukui Prefectural University
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- KATANO Hajime
- Department of Bioscience, Fukui Prefectural University
書誌事項
- タイトル別名
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- Effect of ε-Poly-L-lysine on a Glucose Sensor Based on Glucose Oxidase and Ferricyanide Ion
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<p>ε-Poly-L-lysine (εPL) is a homopolymer of L-lysine residues with linkages between the α-carboxyl and ε-amino groups. εPL exists as a polycationic species under acidic and neutral conditions, and can form a polyion complex with negatively charged glucose oxidase (GOx). We previously reported that εPL significantly promoted a GOx enzymatic reaction using ferricyanide ion ([Fe(CN)6]3−) as the oxidant. Here, we construct a GOx-immobilized electrode using εPL and glutaraldehyde, and show that [Fe(CN)6]3− can efficiently act as the mediator in this electrode. Ferricyanide ion failed to function adequately as the mediator when bovine serum albumin or other polyamines (e.g., polyallylamine, α-poly-L-lysine) were used instead of εPL. The GOx-immobilized electrode using εPL successfully responded to glucose even when the [Fe(CN)6]3− concentration was as low as 1 mM, and exhibited a wide dynamic range of up to several tens of mM. Thus, εPL is considered to be a useful additive for glucose sensors based on the [Fe(CN)6]4−/3−-mediated GOx catalytic current.</p>
収録刊行物
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- Analytical Sciences
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Analytical Sciences 34 (8), 947-951, 2018-08-10
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282763027709824
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- NII論文ID
- 130007432042
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- NII書誌ID
- AA10500785
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 029154028
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- PubMed
- 30101890
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- PubMed
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