Effect of Hinokitiol on Salivary Secretory-IgA Antibody Production System

DOI IR Web Site Open Access
  • KATAOKA Kosuke
    Department of Preventive and Community Dentistry, Faculty of Dentistry, Osaka Dental University
  • KUNITOMO Eiji
    Central R&D Laboratory, Kobayashi Pharmaceutical Co., Ltd.
  • KOBUCHI Kenjiro
    Department of Periodontology, Graduate School of Dentistry, Faculty of Dentistry, Osaka Dental University
  • UENE Masako
    Department of Preventive and Community Dentistry, Faculty of Dentistry, Osaka Dental University
  • DOI Takashi
    Department of Preventive and Community Dentistry, Faculty of Dentistry, Osaka Dental University
  • MIYAKE Tatsuro
    Department of Preventive and Community Dentistry, Faculty of Dentistry, Osaka Dental University

Bibliographic Information

Other Title
  • ヒノキチオールの唾液分泌型IgA抗体産生に与える影響
  • ヒノキチオール ノ ダエキ ブンピガタ IgA コウタイ サンセイ ニ アタエル エイキョウ

Search this article

Abstract

<p> Hinokitiol (HNK) is a well-known antimicrobial and antifungal agent, and is widely used in various formulations including tooth pastes, mouth rinses, aromatics, cosmetics, and food. There is limited information on the immunobiological activity of HNK. In the present study, we investigated the effects of interanasally administered HNK on salivary secretory-IgA antibody (SIgA Ab) secretion in mice. BALB/c (8 weeks old) mice were given 50 μg of HNK four times at weekly intervals (Days 0, 7, 14, and 21) via the nasal route. Saliva samples were collected prior to (−0 hour) and after (0.5, 1.5, 3, and 6 hours) the nasal administration of HNK on respective days, and the levels of salivary SIgA Ab were determined by ELISA. Furthermore, the numbers of SIgA Ab-producing cells (SIgA AFCs) in submandibular glands (SMG) were examined by ELISPOT, and proliferation responses of IgA+ B cells were measured by MTT assays based on the same schedule on Day 21.</p><p> On Days 0, 7, 14, and 21, the maximum level of SIgA Ab secretion in saliva occurred at 1.5 hours after the nasal administration of HNK. Interestingly, the number of doses of HNK and quantities of SIgA secretion showed a positive correlation. On Day 21, there were no significant differences between the number of SMG IgA AFCs prior to and after (0.5, 1.5, 3, and 6 hours) the nasal administration of HNK. However, significantly increased levels of IgA AFCs due to promotion of their proliferative activity were noted 0.5 and 1.5 hours after the nasal administration of HNK. Our results suggest that HNK has an impact on salivary SIgA Ab secretion immediately after nasal administration via elevating the proliferative activity of SMG IgA AFCs.</p>

Journal

Related Projects

See more

Keywords

Details 詳細情報について

Report a problem

Back to top