Flow Injection Chemiluminescence Determination of Neomycin in Pharmaceutical Formulations

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Abstract

A simple flow injection chemiluminescence method was proposed for the determination of neomycin in commercial pharmaceutical formulations. The chemiluminescence reaction is based on the enhancing effect of neomycin on the chemiluminescence reaction between luminol-hydrogen peroxide catalysed by potassium ferrocyanide in the Triton X-100 alkaline media. Neomycin solution was injected into a water carrier stream and then mixed with hydrogen peroxide in the presence of Triton X-100 solutions. The reaction mixture was then merged at a Y-piece with a reagent stream consisting of potassium ferrocyanide and luminol in alkaline solution. The elicited chemiluminescence intensity was measured by a photomultiplier tube operated at a voltage of 880 V. The linear detection range was found to be 0.1–14 μg mL-1. The relative standard deviation for 12 replicates was found to be 2.90% at 4 μg mL-1 of neomycin standard solution with the detection limit of 0.01 μg mL-1, whilst the sample throughput of 152 h-1. The proposed FI-CL method was successfully applied for the determination of neomycin in pharmaceutical formulations. The method validation has been compared versus spectrophotometric method.

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