Electrochemical Study on the Extracellular Electron Transfer Pathway from <i>Shewanella</i> Strain Hac319 to Electrodes

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  • TAKEUCHI Ryosuke
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
  • SUGIMOTO Yu
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
  • KITAZUMI Yuki
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
  • SHIRAI Osamu
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
  • OGAWA Jun
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
  • KANO Kenji
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

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  • Electrochemical Study on the Extracellular Electron Transfer Pathway from Shewanella Strain Hac319 to Electrodes

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Abstract

<p>Shewanella can transfer electrons to various extracellular electron acceptors. We electrochemically investigated the pathway of extracellular electron transfer from Shewanella strain Hac319 to electrodes. A resting cell suspension of Shewanella strain Hac319 containing lactate produced a steady-state sigmoidal wave in the presence of flavin mononucleotide (FMN) in cyclic voltammetry, but not in the absence of FMN. A harvested cell suspension without cell-washing also produced a similar catalytic wave without any external addition of free FMN. The midpoint potentials of the two sigmoidal waves were identical to the redox potential of free FMN. The data indicate that FMN secreted from the Shewanella strain Hac319 works as an electron-transfer mediator from the cell to electrodes. An addition of cyanide to a resting cell suspension of Shewanella strain Hac319 increased the rate of the FMN reduction in the presence of lactate, while it decreased the respiration rate. By considering the fact that cyanide is coordinated to the heme moiety of hemoproteins and shifts the redox potential to the negative potential side, the data indicate that the electron derived from lactate is predominantly transferred in a down-hill mode from an electron donor with a redox potential more negative than that of FMN without going through outer membrane cytochromes c molecules.</p>

Journal

  • Analytical Sciences

    Analytical Sciences 34 (10), 1177-1182, 2018-10-10

    The Japan Society for Analytical Chemistry

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