A novel allele of the ADP-glucose transporter gene in ‘Tanikei QM-1’ markedly increases health-promoting compounds in barley grains

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  • ADP-グルコーストランスポーター遺伝子の新規アリルの同定と複数の機能性成分を高含有するオオムギ品種育成への利用
  • ADP-グルコーストランスポーター イデンシ ノ シンキ アリル ノ ドウテイ ト フクスウ ノ キノウセイ セイブン オ コウガンユウ スル オオムギ ヒンシュ イクセイ エ ノ リヨウ

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Abstract

<p>Barley grains contain high amounts of health-promoting constituents represented by a dietary fiber, β-glucan. In order to further reinforce the health benefits of barley, there have been demands for cultivars containing higher levels of multiple health-promoting compounds. Here, we report that a barley line, ‘Tanikei QM-1’, harbors a novel mutant allele of the Lys5 gene and contains higher amounts of several health-promoting constituents. ‘Tanikei QM-1’, which is derived from the mutagenesis of ‘Shikokuhadaka 97’, and several progeny lines obtained by crossing ‘Tanikei QM-1’ and ‘Hokuriku 48’ or ‘Hokuriku 49’, produces shrunken seeds in which the starch granules and their equatorial grooves show abnormal morphology. Analyses of the health-promoting constituents revealed that the shrunken seed lines contain higher amounts of β-glucan, fructan, arabinoxylan, resistant starch and GABA, and lower starch accumulation in the grains. In particular, β-glucan, fructan and arabinoxylan contents in the lines are higher than those in the high-β-glucan cultivar ‘Beau Fiber’. Conversely, the normal seed progeny lines contain almost the same level of those constituents as the standard cultivars ‘Fiber-Snow’ and ‘Shikokuhadaka 97’. Since the starch granule morphology and reduced starch content in the shrunken seed lines are quite similar to those of ‘Beau Fiber’, which harbors a mutant allele of the Lys5 gene encoding ADP-glucose transporter, we performed a sequence analysis of a 1.5-kbp genomic region of the Lys5 gene and found a novel nonsynonymous substitution common in the shrunken seed lines but not in the normal seed lines. These results strongly suggest that the substitution is the causal mutation for the higher amounts of several health-promoting compounds in the shrunken seed lines. In addition, we developed a dCAPS marker that makes it possible to genotype the mutant allele. The novel allele of the Lys5 gene in ‘Tanikei QM-1’, designated as lys5.i, is expected to provide useful breeding material for the production of barley cultivars with higher health benefits.</p>

Journal

  • Breeding Research

    Breeding Research 20 (2), 124-132, 2018-12-01

    Japanese Society of Breeding

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