Current Status and Issues of Feline Morbillivirus Research :

DOI Open Access
  • FURUYA Tetsuya
    東京農工大学農学部共同獣医学科獣医微生物学研究室
  • ARIKAWA Kaho
    東京農工大学農学部共同獣医学科獣医微生物学研究室
  • Kripitch Sutummaporn
    東京農工大学農学部共同獣医学科獣医微生物学研究室
  • IMURA Yuri
    東京農工大学農学部共同獣医学科獣医微生物学研究室
  • Mahmoud Helal
    東京農工大学農学部共同獣医学科獣医微生物学研究室
  • KIMURA Yusuke
    東京農工大学農学部共同獣医学科獣医微生物学研究室
  • SAEKI Chiharu
    東京農工大学農学部共同獣医学科獣医微生物学研究室
  • MACHIDA Noboru
    東京農工大学農学部共同獣医学科獣医微生物学研究室
  • WACHI Akiko
    東京農工大学農学部共同獣医学科獣医微生物学研究室

Bibliographic Information

Other Title
  • 猫モルビリウイルス研究の現状と課題
  • －病原性の解明に向けて－
  • Toward Understanding of Its Pathogenesis

Search this article

Description

<p>Feline morbillivirus (FmoPV) is a new virus species that was first discovered in Hong Kong in 2012. Study of FmoPV is important because relationship between its infection and tubulointerstitial nephritis in cats was implicated in the first report. We attempted to develop a genetic detection system based on the consensus sequence of 9 FmoPV genomic sequences in the L gene, since there were FmoPV strains undetectable by the previous RT-PCR system. When 83 samples were tested with this system, we detected 4 new FmoPV strains previously undetected by the previous RT-PCR. In order to develop an antibody detection system specific to a FmoPV-antigen, we expressed a recombinant FmoPV P protein in Escherichia coli as a fusion protein with glutathione-S-transferase and developed an ELISA with the purified P protein. We detected FmoPV in 82% (26/34) of formalin-fixed kidney samples from cats suffered from nephritis, suggesting correlation between FmoPV infection and chronic nephritis. We are currently attempting to develop model systems to study FmoPV’s pathogenicity using tissue culture cells or experimental animals.</p>

Journal

Related Projects

See more

Details 詳細情報について

Report a problem

Back to top