Reactivity of Deoxyribonuclease from <i>Streptococcus sanguis I</i>.

DOI
  • Hamada Ikuo
    Department of Microbiology, School of Dentistry, Iwate Medical University
  • Kaneko Masaru
    Department of Microbiology, School of Dentistry, Iwate Medical University

Bibliographic Information

Other Title
  • <i>Streptococcus sanguis</i> I の産生するDeoxyribonuclease の反応性について

Abstract

<p>An extracellular deoxyribonuclease separated from Streptococcus sanguis I destroyed the thymus DNA and then produced mono-, di-, tri-, tetra- and other oligonucleotides.</p><p>These oligonucleotides were determined by the combination of Sephadex G-25 gelfiltration and DEAE-Sephacel ion exchange chromatography in the presence of 7M urea.</p><p>The results were summarized as follows:</p><p>Only thymus DNA was hydrolyzed by the DNase and its molecular weight decreased to 2.2 x 105daltons on 0.8% agarose gel electrophoresis. However, the DNase showed no enzymatic activity for λ phage DNA, fd phage DNA, thermally denatured thymus DNA or themally denatured thymus λDNA.</p><p>It suggests that the DNase has high specific activity for native thymus DNA.</p>

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Keywords

Details 詳細情報について

  • CRID
    1390282763083022464
  • NII Article ID
    130007599324
  • DOI
    10.20663/iwateshigakukaishi.8.1_29
  • ISSN
    24241822
    03851311
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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