The Metagenome Approach: A New Resource for Glycosidases

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  • Matsuzawa Tomohiko
    Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)

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  • 糖質加水分解酵素の新しいリソースとしてのメタゲノム
  • トウシツ カスイブンカイ コウソ ノ アタラシイ リソース ト シテ ノ メタゲノム

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Abstract

<p>Glycosidases play vital roles in the degradation of oligo- and polysaccharides. To date, many glycosidases have been isolated from microorganisms, plants, and animals, and have been used in industrial applications. Although microorganisms are important resources of novel and useful glycosidases, we are currently unable to culture most microorganisms in the laboratory. The metagenomic approach, which is a culture-independent method used to obtain genomic information regarding environmental microorganisms, is a powerful tool for the isolation of genes encoding novel glycosidases from microorganisms. This review introduces unique characters and biotechnological applications of β-glucosidases and a β-xylosidase/α-L-arabinofuranosidase isolated with the metagenomic approach.</p>

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