A MICRODISSECTION METHOD FOR DETECTING THORACIC VISCERAL MALFORMATIONS IN MOUSE AND RAT FETUSES

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  • マウスおよびラット胎仔の胸部内蔵奇形観察のための顕微解剖法

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Recently, in addition to gross and skeletal anomalies, visceral types have been examined in teratological studies using chemicals with fetal mice and rats, and Wilson's method has widely been used. However, the technique presents a disadvantage in that it is difficult to detect certain cardiac anomalies because of the cross sectioning of the thoracic area. Barrow and Taylor have suggested a rapid microdissection method for complete cardiac examination characterized by sagittal sectioning of the heart. Sagittal sectioning is more suitable for cardiac evaluation, but it is still not an efficient means to detect defects of the interventricular septum and atrioventricular valves or malposition of the major arteries. The present technique is a modification of Barrow and Taylor's. It embraces an inspection of the back side of all the thoracic viscera with the exception of the lungs after removing the organs from the pleural cavity, and utilizes a transverse section a little below the base of the heart. This technique and the following thoracic visceral malformations, produced mainly by chlorambucil in rat fetuses, are presented: dysplasia of the thymus and lung, diaphragmatic hernia, dextrorotation or levorotation of the heart, retroesophageal right subclavian artery, interruption of the aortic arch, coarctation of the aorta, transposition of the arterial trunks, persistent truncus arteriosus, vascular ring, right aortic arch, supernumerary coronay orifice, bicuspid aortic or pulmonic valve, VSD, common atrioventricular orifice, bifid anterior cusp of the mitral valve, mitral or tricuspid stenosis, double inferior venae cavae, and hypoplastic azygos vein.

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