A semi-nested RT-PCR assay for detection of norovirus in rat fecal samples

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  • Moreira Josélia Cristina de Oliveira
    Cidade Universitária “Zeferino Vaz” Barão Geraldo, 250, 5 de Junho Street, Campinas - São Paulo 13083-970, Brazil
  • Rodrigues Daniele Masselli
    Cidade Universitária “Zeferino Vaz” Barão Geraldo, 250, 5 de Junho Street, Campinas - São Paulo 13083-970, Brazil
  • Gilioli Rovilson
    Cidade Universitária “Zeferino Vaz” Barão Geraldo, 250, 5 de Junho Street, Campinas - São Paulo 13083-970, Brazil
  • Costa Fernando Ferreira
    Cidade Universitária “Zeferino Vaz” Barão Geraldo, 480 Carlos Chagas Street, Campinas - São Paulo 13083-878, Brazil
  • Albuquerque Dulcinéia Martins
    Cidade Universitária “Zeferino Vaz” Barão Geraldo, 480 Carlos Chagas Street, Campinas - São Paulo 13083-878, Brazil

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抄録

<p>Norovirus is a highly prevalent pathogen that can infect a wide range of host species. Thus far, there have only been two reports of norovirus infection in rats. Diagnostic assays for the detection of norovirus are well established, but a specific molecular assay for the diagnosis of norovirus infection in laboratory rats has not yet been reported. In this study, we describe the development of a sensitive, semi-nested RT-PCR assay for detection of norovirus in fecal samples from Rattus norvegicus, reared in animal facilities under different sanitary barrier conditions. Additionally, we describe the first report of the presence of norovirus in rat colonies from Brazilian animal facilities.</p>

収録刊行物

  • Experimental Animals

    Experimental Animals 68 (2), 169-176, 2019

    公益社団法人 日本実験動物学会

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