Molecular Detection and Characterization of <i>p44</i>/<i>msp2</i> Multigene Family of <i>Anaplasma phagocytophilum</i> from <i>Haemaphysalis longicornis</i> in Mie Prefecture, Japan

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  • Su Hongru
    Laboratory of Microbiology, Graduate Program in Pharmaceutical and Nutritional Sciences, Integrated Graduate School of Pharmaceutical and Nutritional Sciences, University of Shizuoka
  • Sato Ayaka
    Laboratory of Microbiology, Graduate Program in Pharmaceutical and Nutritional Sciences, Integrated Graduate School of Pharmaceutical and Nutritional Sciences, University of Shizuoka
  • Onoda Eri
    Laboratory of Microbiology, Graduate Program in Pharmaceutical and Nutritional Sciences, Integrated Graduate School of Pharmaceutical and Nutritional Sciences, University of Shizuoka
  • Fujita Hiromi
    Laboratory of Microbiology, Graduate Program in Pharmaceutical and Nutritional Sciences, Integrated Graduate School of Pharmaceutical and Nutritional Sciences, University of Shizuoka Mahara Institute of Medical Acarology
  • Sakabe Shigetoshi
    Department of Medicine and Infectious Disease, Ise Red Cross Hospital
  • Akachi Shigehiro
    Mie Prefecture Health and Environment Research Institute
  • Oishi Saori
    Department of Microbiology, Shizuoka Institute of Environment and Hygiene
  • Abe Fuyuki
    Department of Microbiology, Shizuoka Institute of Environment and Hygiene
  • Kanda Takashi
    Department of Microbiology, Shizuoka Institute of Environment and Hygiene
  • Shimamura Yuko
    Laboratory of Microbiology, Graduate Program in Pharmaceutical and Nutritional Sciences, Integrated Graduate School of Pharmaceutical and Nutritional Sciences, University of Shizuoka
  • Masuda Shuichi
    Laboratory of Microbiology, Graduate Program in Pharmaceutical and Nutritional Sciences, Integrated Graduate School of Pharmaceutical and Nutritional Sciences, University of Shizuoka
  • Ohashi Norio
    Laboratory of Microbiology, Graduate Program in Pharmaceutical and Nutritional Sciences, Integrated Graduate School of Pharmaceutical and Nutritional Sciences, University of Shizuoka

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Other Title
  • Molecular Detection and Characterization of p44/msp2 Multigene Family of Anaplasma phagocytophilum from Haemaphysalis longicornis in Mie Prefecture, Japan

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Abstract

<p>Anaplasma phagocytophilum is an obligate intracellular bacterium that causes human granulocytic anaplasmosis (HGA), an emerging tick-borne infectious disease. This bacterium expresses various 44-kDa major outer membrane proteins encoded by the p44/msp2 multigene family to avoid the host immune system. We previously detected A. phagocytophilum p44/msp2 from the tick Haemaphysalis longicornis in Mie Prefecture, Japan in 2008. In this study, we further investigated a total of 483 H. longicornis ticks (220 adults and 263 nymphs) collected from the Mie Prefecture by PCR targeting p44/msp2 to characterize the p44/msp2 multigene family of A. phagocytophilum. Six of the 483 ticks tested were PCR-positive for A. phagocytophilum p44/msp2, and these positive individuals were at the nymph stage of the tick life cycle. Cloning, sequencing, and phylogenetic analyses of the amplicons revealed that the 11 p44/msp2 clones obtained from the positive ticks shared a 54.9%–99.3% amino acid sequence similarity with the 27 previously identified clones from HGA patients in Japan. In particular, 6 p44/msp2 clones displayed the highest similarities (97.2%–99.3%) with 3 previously identified clones (FJ417343, FJ417345, FJ417357). Thus, the data from this study provide important public health information regarding A. phagocytophilum infection transmitted by H. longicornis ticks, especially at the nymph stage.</p>

Journal

  • Japanese Journal of Infectious Diseases

    Japanese Journal of Infectious Diseases 72 (3), 199-202, 2019

    National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

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