Double-strand breaks in genome-sized DNA caused by photo-irradiation, gamma-rays and ultrasound
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- Kenmotsu Takahiro
- Faculty of Life and Medical Sciences, Doshisha University
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- Ma Yue
- Faculty of Life and Medical Sciences, Doshisha University
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- Usui Moe
- Faculty of Life and Medical Sciences, Doshisha University
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- Yoshikawa Yuko
- Faculty of Life and Medical Sciences, Doshisha University
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- Yoshikawa Kenichi
- Faculty of Life and Medical Sciences, Doshisha University
説明
<p>We evaluated double-strand breaks (DSBs) in genome-sized DNA (T4 DNA; 166 kbp) caused by photo-irradiation, gamma-rays and ultrasound through a single DNA observation by fluorescence microscopy in a quantitative manner. Based on experimental results, DSBs were induced by two-step mechanism for the photo-irradiation. On the other hands, one-step mechanism led to DSBs for the irradiations of gamma-ray and ultrasound. Regarding the effect of ultrasound, it was found that DSBs were generated above a threshold power. It is suggested that the experimental methodology of single DNA observation serves as a useful tool for studying DSBs of genome-sized DNA.</p>
収録刊行物
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- 日本シミュレーション学会英文誌
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日本シミュレーション学会英文誌 6 (1), 189-194, 2019
一般社団法人 日本シミュレーション学会
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詳細情報 詳細情報について
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- CRID
- 1390282763120217728
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- NII論文ID
- 130007628695
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- ISSN
- 21885303
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
- OpenAIRE
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- 抄録ライセンスフラグ
- 使用不可
