Isolation of Nervous Necrosis Virus from Hybrid Grouper (<i>Epinephelus fuscoguttatus</i>♀ × <i>Epinephelus lanceolatus</i>♂) Cultured in Guangxi, China

DOI Web Site Web Site 11 References
  • Yu Qing
    Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry, Guangxi Academy of Sciences
  • Liu Mingzhu
    Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry, Guangxi Academy of Sciences
  • Wei Shina
    College of Marine Sciences, South China Agricultural University
  • Xiao Hehe
    College of Life Science, Henan Normal University
  • Wu Siting
    Guangxi Key Lab for Marine Biotechnology, Guangxi Institute of Oceanography
  • Qin Xianling
    Key Laboratory of Marine Environmental Science, Guangxi Academy of Sciences
  • Shi Deqiang
    Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry, Guangxi Academy of Sciences
  • Li Siqiao
    Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry, Guangxi Academy of Sciences
  • Wang Taixia
    College of Life Science, Henan Normal University
  • Li Pengfei
    Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry, Guangxi Academy of Sciences

Bibliographic Information

Other Title
  • Isolation of Nervous Necrosis Virus from Hybrid Grouper (Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus♂) Cultured in Guangxi, China

Search this article

Abstract

<p>Outbreaks of suspected viral nervous necrosis emerged in cultured groupers in Guangxi, China in August 2018. ​Histopathology showed severe vacuolation in the brain tissue of the diseased juvenile hybrid grouper (Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus♂). ​A nervous necrosis virus (NNV) was isolated from pooled sample of the brain and eyes using a grouper cell line EAGB. ​Cytopathic effect with multiple cytoplasmic vacuolation cells developed in EAGB cells. ​The coat protein gene sequence of the isolate (GNNV-Gx) showed that it belonged to RGNNV genotype and was most closely related to that of a tiger grouper NNV registered in GenBank. ​The infectivity of the isolate decreased remarkably at pH 3 and pH 10 and was susceptible to 60°C.</p>

Journal

  • Fish Pathology

    Fish Pathology 54 (1), 16-19, 2019-03-15

    The Japanese Society of Fish Pathology

References(11)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top