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- 神鳥 和彦
- 大阪教育大学教育学部理科教育講座
書誌事項
- タイトル別名
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- Synthesis of Positively Charged Calcium Hydroxyapatite Nano-Crystals and Their Adsorption Behavior of Proteins
説明
<p>Positively charged calcium hydroxyapatite [Ca10(PO4)6(OH)2 ; Hap] nano-crystals were prepared by using β-alanine and clarified the adsorption affinity of these surface amide functionalized Hap nanocrystals to proteins. Colloidal surface amide functionalized Hap nano-crystals were prepared by wet method in the presence of various amounts of β-alanine by changing molar ratio of β-alanine/Ca (β/Ca ratio) in the solution. The rod-like nano-crystals were lengthened with addition of β-alanine though their width did not vary; carboxyl groups of β-alanine are strongly coordinated to Ca2+ ions exposed on ac and/or bc faces to inhibit particle growth to a- and/or β-axis directions and enhance the particle growth along to the c-axis. No difference can be recognized on the crystal structure among the synthesized Hap nano-crystals by XRD measurements. However, the large difference was recognized by TG-DTA and FTIR measurements. Those measurements reveled that β-alanine is incorporated on the β-alanine-Hap nano-crystal surface up to the β/Ca ratio of 1.0, though they are absent in the nano-crystals synthesized at β/Ca ratio>-2.0. The zeta potential (ZP) of β-alanine-Hap nano-crystals prepared at β/Ca =0.4 and 1.0 of those incorporating β-alanine exhibited positive charge at pH<-5.9. The saturated amounts of adsorbed BSA for the positively charged β-alanine-Hap nano-crystals were increased 2.3~2.4-fold by their electrostatic attraction force between positively charged β-alanine-Hap nano-crystals and negatively charged BSA molecules. We were able to control the adsorption affinity of Hap nano-crystal by changing their surface charge.</p>
収録刊行物
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- ホソカワ粉体工学振興財団年報
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ホソカワ粉体工学振興財団年報 16 (0), 97-101, 2008
公益財団法人 ホソカワ粉体工学振興財団
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詳細情報 詳細情報について
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- CRID
- 1390282763124091008
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- NII論文ID
- 130007667349
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- ISSN
- 21894663
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用可