Cryo-EM System for Supporting Drug Discovery and Life Sciences

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Other Title
  • 創薬等ライフサイエンス用クライオ電子顕微鏡単粒子解析支援システム
  • ソウヤク トウ ライフサイエンスヨウ クライオ デンシ ケンビキョウタンリュウシ カイセキ シエン システム

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Abstract

<p>Recently, electron cryo-microscopy single particle analysis (cryo-EM SPA) has been developed dramatically and can be used for drug design because of the greatly improved resolution to near-atomic level. Nowadays, based on the atomic structures of drugs within the target protein molecules, the drugs can be designed or modified more efficiently, which is called as structure-based drug design (SBDD). We have constructed a cryo-EM system for supporting collaborations for drug discovery and life sciences, mainly supported by and used for national platform projects of BINDS and PDIS, AMED. For example, structures of a virus-like particle of PfV (Pyrococcus furiosus virus-like particle) of 30 nm in diameter have been determined at near-atomic resolutions (3.3 Å) by both conventional cryo-EM (CTEM) and Volta phase contrast cryo-EM (VPC-TEM) SPA methods within a week. In addition to the high-throughput structure analysis, we succeeded high-resolution cryo-EM SPA at 2.3 Å resolution with an advanced direct electron detecting camera, Falcon 3EC in an electron counting mode. Here, we introduce our cryo-EM system for the high-throughput and high-resolution single particle analyses.</p>

Journal

  • KENBIKYO

    KENBIKYO 53 (1), 8-12, 2018-04-30

    The Japanese Society of Microscopy

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