Development of a direct competitive enzyme-linked immunosorbent assay for determination of the fungicide mepanipyrim and its metabolite

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  • Miyake Shiro
    Advanced Science, Technology & Management Research Institute of Kyoto Research & Development Division, Horiba, Ltd. Azabu University
  • Hirakawa Yuki
    Advanced Science, Technology & Management Research Institute of Kyoto Kyoto Women’s University
  • Yamasaki Tomomi
    Advanced Science, Technology & Management Research Institute of Kyoto Kyoto Women’s University
  • Watanabe Eiki
    Institute for Agro-Environmental Sciences, National Agriculture and Food Research Organization
  • Harada Ayako
    Toyohashi University of Technology
  • Adachi Kayo
    Aichi Science & Technology Foundation
  • Iwasa Seiji
    Toyohashi University of Technology
  • Narita Hiroshi
    Kyoto Women’s University

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<p>A direct competitive enzyme-linked immunosorbent assay (dc-ELISA) was developed for determination of anilinopyrimidine fungicide mepanipyrim in vegetables. Two derivatives of mepanipyrim and mepanipyrim propanol type metabolite which carried carboxy acid were synthesized and conjugated with keyhole limpet hemocyanin. BALB/c mice were immunized to prepare anti-mepanipyrim monoclonal antibodies (MoAbs) by obtained conjugates. The dc-ELISAs based on the prepared MoAbs, MPP107 and MPP204, showed working ranges between 0.12 and 1.8 ng/mL with mepanipyrim for MPP107, 0.12 and 2.4 ng/mL with mepanipyrim for MPP204, and 0.2 ng/mL and 5.7 ng/mL with the mepanipyrim propanol type for MPP204. The dc-ELISAs showed the sufficient sensitivity to determine the mepanipyrim residues for the MRLs of 1–15 mg/kg among the majority of vegetables and fruits in Japan. Recovery and/or correlation results from HPLC suggested that the dc-ELISAs would be applicable to the residue analysis of mepanipyrim and its propanol type in vegetables.</p>

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