3D Visualization of Osteogenic Capillaries in Mineralized Bone Tissue by Integrating Micro-CT with Light Sheet Fluorescence Microscopy Imaging

DOI Web Site
  • Miya Ayako
    慶應義塾大学医学部共同利用研究室（中央機器管理部門）
  • Kuroda Yukiko
    慶應義塾大学医学部細胞組織学研究室
  • Takeda Yoshihiro
    株式会社リガクX線研究所　ナノ形状計測グループ
  • Matsuo Koichi
    慶應義塾大学医学部共同利用研究室（中央機器管理部門） 慶應義塾大学医学部細胞組織学研究室

Bibliographic Information

Other Title
  • マイクロCT像とLight sheet蛍光顕微鏡像の融合による骨形成性毛細血管の3D可視化
  • マイクロ CTゾウ ト Light sheet ケイコウ ケンビキョウゾウ ノ ユウゴウ ニ ヨル ホネ ケイセイセイ モウサイ ケッカン ノ 3D カシカ

Search this article

Abstract

<p>Bone microstructure morphology can be visualized in three dimensions by X-ray computed tomography (CT) using high resolution X-ray microscopes.Light sheet fluorescence microscopy (LSFM), or multi-angle fluorescence microscopy, also allows whole-mount three-dimensional imaging of fluorescently-labeled molecules rather than X-rays. Integration of spatial information based on both X-rays and fluorescence would allow one to visualize the location of fluorescent molecules within bone. Here, we focused on morphology of the malleus, the auditory ossicle that attaches to the tympanic membrane. In mice, the malleus contains abundant “osteogenic capillaries” during postnatal endochondral ossification. To visualize those capillaries, we perfused mice with fluorescence-labeled tomato-lectin to mark vessels and then performed micro-CT scanning, decalcification, and LSFM imaging. We then compared canal structures observed using micro-CT with the network of blood capillaries visualized using tomato-lectin. By integrating images obtained by applying two different principles of three-dimensional reconstruction, we showed that intra-osseous blood capillaries can be superimposed within regions lacking bone in the malleus. These methods will be discussed here with future perspectives.</p>

Journal

  • KENBIKYO

    KENBIKYO 51 (2), 88-93, 2016-08-30

    The Japanese Society of Microscopy

Related Projects

See more

Keywords

Details 詳細情報について

Report a problem

Back to top