Technique for the Production of Cultured Color-Matched Epithelial Sheets for Application in Cosmetics

DOI
  • Takeuchi Takayuki
    Department of Plastic and Reconstructive Surgery, St. Marianna University School of Medicine
  • Kajikawa Akiyoshi
    Department of Plastic and Reconstructive Surgery, St. Marianna University School of Medicine
  • Sumie Rena
    Department of Plastic and Reconstructive Surgery, St. Marianna University School of Medicine
  • Nabeshima Ryota
    Department of Plastic and Reconstructive Surgery, St. Marianna University School of Medicine
  • Miyano Ryotaro
    Department of Plastic and Reconstructive Surgery, St. Marianna University School of Medicine
  • Ichida Mio
    Department of Plastic and Reconstructive Surgery, Division of Stem Cell Medicine (Endowed Chair by ANGFA Co.), St. Marianna University School of Medicine
  • Tateshita Toru
    Department of Plastic and Reconstructive Surgery, St. Marianna University School of Medicine
  • Inoue Hajime
    Department of Plastic and Reconstructive Surgery, Division of Stem Cell Medicine (Endowed Chair by ANGFA Co.), St. Marianna University School of Medicine

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Other Title
  • 培養表皮の色素調節技術の開発
  • 整容的応用のための基礎的検討

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<p>The transplantation of cultured epithelium is useful in the treatment of large-area skin defects, but such epithelium can also be cultured simultaneously with melanocytes in the culture process. By using this technique, therapy can be performed to adjust pigmentation although free adjustment of the color tone is not possible.<br/>In this study, the authors attempted to develop a culture technique for coloring cultured epithelium that allows color matching by controlling the number and function of melanocytes in the cultured keratinocytes.<br/>Cocultivation of keratinocytes and melanocytes was possible by the method of Rheinwald and Green.<br/>However, endothelin-1, which promotes melanocyte proliferation, did not enhance the proliferation of these cells in this culture condition.<br/>Dinoprost, a promotor of melanin synthesis, did not affect proliferation but did enhance melanin synthesis.<br/>During keratinocyte culture, melanocytes specifically cocultured by another technique, were added at the time of passaging of the cultured keratinocytes, thus allowing simultaneous culture of keratinocytes and melanocytes.<br/>In addition, the melanocytes coexisted at a uniform density on the surface of the cultured keratinocytes.<br/>Depending on the concentration of the applied melanocyte cells, the dopa response of the cultured keratinocytes was enhanced. From the above, it was possible to culture melanocytes under different specific conditions in advance during keratinocytes culture, and the color tone of the cultured keratinocytes could be freely changed by application of the coexisting cultured melanocytes during preparation of cultured epithelium for transplantation.<br/>Pharmaceutical control was also possible by adding a melanin synthesis promoter such as dinoprost.<br/>Depigmentation and pigmentation can be a major esthetic afflictions in patients. This technology may be applicable to color matching of transplantion sites such as in vitiligo.</p>

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