An Investigation of Osteogenesis on Ultraviolet Light-treated Surfaces Using a Type 2 Diabetes Rat Model

DOI
  • FUNATO Akiyoshi
    Department of Oral Pathology, School of Dentistry, Aichi Gakuin University
  • KOMATSU Shinichi
    Department of Oral Pathology, School of Dentistry, Aichi Gakuin University
  • HOTTA Hisato
    Department of Oral Pathology, School of Dentistry, Aichi Gakuin University
  • SUGITA
    Department of Oral Pathology, School of Dentistry, Aichi Gakuin University Center of Advanced Oral Science, Aichi Gakuin University
  • WADA Akira
    Department of Oral Pathology, School of Dentistry, Aichi Gakuin University
  • TAKEUCHI Kazuo
    Department of Gerodontology, School of Dentistry, Aichi Gakuin University
  • KUBO Katsutoshi
    Department of Oral Pathology, School of Dentistry, Aichi Gakuin University Center of Advanced Oral Science, Aichi Gakuin University
  • MAEDA Hatsuhiko
    Department of Oral Pathology, School of Dentistry, Aichi Gakuin University Center of Advanced Oral Science, Aichi Gakuin University

Bibliographic Information

Other Title
  • 紫外線照射処理チタン上で培養した2型糖尿病モデルラットの骨髄由来骨芽細胞様細胞の分化に関する研究

Abstract

<p>One of the risk factors of implant treatment is patients with diabetes mellitus, as it is difficult to acquire osseointegration compared with healthy patients. There are time-related changes to the titanium surface, and the cell affinity and histocompatibility of the titanium decreases. Ultraviolet (UV) treatment to titanium is known to effectively improve these changes. Therefore, in this study, we investigated whether implant treatment for diabetic patients could be enhanced by UVtreating titanium surfaces cultured with bone marrow-derived osteoblast-like cells of type 2 diabetic rats (Spontaneously Diabetic Torii : SDT). These cells were cultured in a low glucose environment similar to the state in which the blood glucose level was controlled.</p><p>The experiment was divided into two groups : 1) The control group in which osteoblast-like cells of SDT rats were placed on control discs and 2) the UV group in which osteoblast-like cells of SDT rats were placed on UV-treated discs. In this study, these osteoblastic cells were derived from SDT rat bone marrow which was cultured in a low glucose environment, and placed onto both the control and UV treated discs. The control discs were used 4 weeks after surface acid-etching treatment. The UV-treated discs were subjected to 48 hours of UV irradiation. In addition, WST-1 Cell Counting Kit, alkaline phosphatase (ALP) activity and Alizarin red staining were used to evaluate t he number of attached cells, differentiation, and mineralization, respectively.</p><p>In the UV group, the number of attached cells was small compared with the control group. The ALP activity of the UV group was higher than that of the control group. Furthermore, calcification activity of the UV group was higher than that of the control group.</p><p>Collectively, our data suggest that the osteoblast-like cells of SDT rats on the UV-treated discs enhanced differentiation to osteoblasts. It is considered that UV treatment of titanium is a useful method for implant treatment of diabetic patients. However, further study is required to examine the differentiation of osteoblasts and expression of bone formation related genes in a high glucose environment, in addition to further in vivo research of implants for diabetic rats.</p>

Journal

Details 詳細情報について

  • CRID
    1390283659847436544
  • NII Article ID
    130007792286
  • DOI
    10.11237/jsoi.32.339
  • ISSN
    21879117
    09146695
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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