A Flow Injection Chemiluminescence Method for the Determination of Retinol in Pharmaceutical Formulations by Using Luminol-Diperiodatoargentate(III) Reaction

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  • AMBAR
    Department of Chemistry, University of Balochistan
  • ASGHAR Muhammad
    Department of Chemistry, University of Balochistan
  • AMEEN Fuad
    Department of Botany & Microbiology, Faculty of Science, King Saud University
  • AL-NADHARI Saleh
    Plant Protection Department, College of Food and Agricultural Sciences, King Saud University
  • WASEEM Amir
    Department of Chemistry, Quaid-i-Azam University
  • YAQOOB Mohammad
    Department of Chemistry, University of Balochistan
  • ALARFAJ Abdullah A.
    Department of Botany & Microbiology, College of Science, King Saud University
  • NABI Abdul
    Department of Chemistry, University of Balochistan

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Abstract

<p>In this paper, a chemiluminescence (CL) method is proposed for retinol determination by combining flow injection (FI) methodology. The CL reaction is based on the oxidation of luminol by diperiodatoargentate(III) (DPA) in the presence of retinol. Under the optimum conditions, the relative CL intensity was linear to the concentration of retinol over the range 5.0×10−3-14 mg L−1 (y=347.26x+2.5944, R2=0.9999, n=8) with limit of detection (LOD) of 1.5×10−3 mg L−1 (S/N=3) and limit of quantification (LOQ) of 5.0×10−3 mg L−1 (S/N=10). The relative standard deviation (RSD) was from 1.04-3.4% over the range studied and injection throughputs of 150 h−1. The method was satisfactorily applied to retinol in pharmaceutical formulation samples. The samples were saponified and extracted with liquid-liquid extraction using ether as an extractant. The possible CL mechanism is supported by CL and UV-visible spectrophotometric studies.</p>

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