Synthesis of One Double Bond-Inserted Retinal Analogs and Their Binding Experiments with Opsins: Preparation of Novel Red-Shifted Channelrhodopsin Variants
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- Okitsu Takashi
- Laboratory of Organic Chemistry for Life Science, Kobe Pharmaceutical University
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- Yamano Yumiko
- Laboratory of Organic Chemistry for Life Science, Kobe Pharmaceutical University
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- Shen Yi-Chung
- Department of Biophysics, Graduate School of Science, Kyoto University
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- Sasaki Toshikazu
- Department of Biophysics, Graduate School of Science, Kyoto University
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- Kobayashi Yuka
- Laboratory of Organic Chemistry for Life Science, Kobe Pharmaceutical University
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- Morisawa Shoko
- Laboratory of Organic Chemistry for Life Science, Kobe Pharmaceutical University
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- Yamashita Takahiro
- Department of Biophysics, Graduate School of Science, Kyoto University
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- Imamoto Yasushi
- Department of Biophysics, Graduate School of Science, Kyoto University
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- Shichida Yoshinori
- Research Organization of Science and Technology, Ritsumeikan University
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- Wada Akimori
- Laboratory of Organic Chemistry for Life Science, Kobe Pharmaceutical University
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Abstract
<p>In optogenetics, red-shifted channelrhodopsins (ChRs) are eagerly sought. We prepared six kinds of new chromophores with one double bond inserted into the polyene side chain of retinal (A1) or 3,4-didehydroretinal (A2), and examined their binding efficiency with opsins (ReaChR and ChrimsonR). All analogs bound with opsins to afford new ChRs. Among them, A2-10ex (an extra double bond is inserted at the C10–C11 position of A2) showed the greatest red-shift in the absorption spectrum of ChrimsonR, with a maximum absorbance at 654 nm (67 nm red-shifted from that of A1-ChrimsonR). Moreover, a long-wavelength spectral boundary of A2-10ex-ChrimsonR was extended to 756 nm, which reached into the far-red region (710–850 nm).</p>
Journal
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- Chemical and Pharmaceutical Bulletin
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Chemical and Pharmaceutical Bulletin 68 (3), 265-272, 2020-03-01
The Pharmaceutical Society of Japan
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Details 詳細情報について
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- CRID
- 1390283659855385472
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- NII Article ID
- 130007804067
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- NII Book ID
- AA00602100
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- ISSN
- 13475223
- 00092363
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- NDL BIB ID
- 030275122
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- PubMed
- 32115534
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed