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- Kawasaki Moe
- Dept. Genomic Neurology., IMEG., Kumamoto Univ.
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- Asamitsu Sefan
- Dept. Genomic Neurology., IMEG., Kumamoto Univ.
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- Shioda Norifumi
- Dept. Genomic Neurology., IMEG., Kumamoto Univ.
Bibliographic Information
- Other Title
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- マウス脳におけるRNAグアニン四重鎖の同定と神経機能における役割
Abstract
<p>G-quadruplexes (G4) are noncanonical four-stranded nucleic acid structures formed by guanine-rich sequences. Recently, a number of studies have demonstrated that RNAs containing G4 structures are involved in biological and pathological processes, including transcription, mRNA maturation, translation, and their relevance to G4-binding proteins. However, the detail function of G4 RNAs in neuron still elusive. In this study, to identify G4 RNAs in brain, we performed an RNA immunoprecipitation sequencing (RIP-seq) using a G4-specific antibody, named G4 RIP-seq. We found several mRNAs of genes that are likely relevant to neuronal function or diseases. In fact, those mRNA has several G-rich sequence motifs and we successfully determined 3D G4 structures of those motifs in vitro. Immunochemical assays of certain G4-binding protein, together with in situ hybridization of mRNAs suggested that cellular G4 RNA might be relevant to neuronal or neuro-physical function. In this presentation, we will discuss the recent results of this study.</p>
Journal
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- Proceedings for Annual Meeting of The Japanese Pharmacological Society
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Proceedings for Annual Meeting of The Japanese Pharmacological Society 93 (0), 3-O-098-, 2020
Japanese Pharmacological Society
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Details 詳細情報について
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- CRID
- 1390283659859588224
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- NII Article ID
- 130007811836
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- ISSN
- 24354953
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- Text Lang
- ja
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed