Study on the role of moonlighting proteins in resistance of cadmium and mercury of lactic acid bacteria

Kinoshita Hideki, Morishita Koki, Murata Yutaro, Koga Fumiya, Yasuda Shin, Araki Tomohiro, Igoshi Keiji

doi:10.11465/milk.69.11

<p> In this study, the role of moonlighting proteins (MP) in heavy metals resistance of lactic acid bacteria were investigated. In the enzyme activity test of cell surface of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in 20 strains, Lactobacillus casei and L. paracasei showed high enzyme activities. The proteins of three strains extracted with distilled water (DW) and phosphate buffered saline (PBS) were detected by SDS-PAGE. Several bands were observed around 40 kDa and 66 kDa in PBS washing, but almost no protein was detected by DW washing. Moreover, no sugar was detected by phenol-sulfuric acid method. The 40 kDa protein of L. rhamnosus TOKAI 45m and L. casei (or L. paracasei) TOKAI 83m was identified as GAPDH, and the 66 kDa protein of TOKAI 45m and TOKAI 65m strains was identified as GroEL. TOKAI 65m and TOKAI 83m strains showed lower cadmium biosorption in PBS-washed cells than in DW-washed cells, but TOKAI 45m strain showed the opposite results. TOKAI 83m strain had a lower mercury biosorption with PBS washing although no difference in TOKAI 45m and TOKAI 65m strains. In cadmium resistance test, there was almost no difference in TOKAI 65m strain, but PBS-washed cells were less resistant in the other two strains. Interestingly, mercury showed the opposite trend to cadmium. These results suggest that MP may protect bacterial cells from cadmium toxicity whereas MP may promote mercury uptake and toxicity at least TOKAI 83m strain. This study strongly suggested that MP might affect heavy metal resistance.</p>

Study on the role of moonlighting proteins in resistance of cadmium and mercury of lactic acid bacteria

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