Rapid Counting Method for Bacterial Spores Using Hydrostatic Pressure-induced Germination

SHIGETA Yujin, NAKANISHI Mikako, MASTUBARA Masahiro, SHIONO Tadahiko, MATSUSHITA Rie, HASHIMOTO Akihiko, AOYAMA Yasushi

doi:10.34470/hitriftrc.1.1_13

Bibliographic Information

Other Title

圧力発芽誘導を利用した芽胞数の迅速測定技術

Abstract

<p>Staining with 5(6)-Carboxyfluorescein diacetate (CFDA) is widely used as a rapid counting method for viable bacteria. However, bacterial spores are not stained by CFDA. We studied a method of staining bacterial spores using CFDA after hydrostatic pressure-induced germination. The B.subtilis spores were stained using CFDA by pressurization at 50 MPa or more. The stained spore counts by fluorescence microscopy were almost equivalent to viable counts measured in the cultivation method. Twelve types of Bacillus spores and Geobacillus stearothermopilus spores were measured using this method. The difference of the measured values between the staining method and the cultivation method was within one log-cycle. We also inoculated B.subtilis spores in a liquid seasoning and measured the counts after hydrostatic pressure-induced germination (100 MPa, 40°C, 15 min) using a commercially available rapid measurement device. The correlation coefficient of the rapid method and the cultivation method was 0.99. The results suggested that the CFDA staining method using pressurization may be used for rapid counting of bacterial spores.</p>

Journal

Bulletin of Hiroshima Prefectural Technology Research Institute Food Technology Research Center

Bulletin of Hiroshima Prefectural Technology Research Institute Food Technology Research Center 29 (0), 13-18, 2020-03-16

Hiroshima Prefectural Technology Research Institute Food Technology Research Center

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Details 詳細情報について

CRID: 1390285300156269952

NII Article ID: 130007840488

DOI: 10.34470/hitriftrc.1.1_13

ISSN: 24355909; 18838324

Text Lang: ja

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JaLC
CiNii Articles

Abstract License Flag: Disallowed

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