Production of Large-volume Cellulase Crystals for Visualization of Hydrogen Atoms

DOI 1 Citations Open Access

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Other Title
  • 水素原子の可視化を目指したセルラーゼの大型結晶作製

Abstract

Water molecules play important roles in enzymatic reaction especially for hydrolases, which use water as a substrate. Neutron crystallography is a powerful tool for visualizing hydrogen atoms to understand protonation states of protein and orientation of water molecules, while it requires a large-volume crystal (>1 mm3 ) compared with X-ray crystallography. To overcome the hurdle of crystal volume, a counter diffusion device equipped with 2 mm diameter can be used at the microgravity experiments. We report here the crystallization of two cellulases which hydrolyse glycosidic linkages in cellulose.

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Details

  • CRID
    1390285300157612544
  • NII Article ID
    130007842594
  • DOI
    10.15011//jasma.34.340108
  • ISSN
    21889783
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
    • KAKEN
    • Crossref
  • Abstract License Flag
    Disallowed

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