Production of Large-volume Cellulase Crystals for Visualization of Hydrogen Atoms
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- TACHIOKA Mikako
- Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- NAKAMURA Akihiko
- Graduate School of Agricultural and Life Sciences, The University of Tokyo Institute for Molecular Science, National Institute of Natural Science
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- ISHIDA Takuya
- Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- TAKAHASHI Sachiko
- Confocal Science Inc.,
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- YAN Bin
- Confocal Science Inc.,
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- TANAKA Hiroaki
- Confocal Science Inc.,
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- FURUBAYASHI Naoki
- Maruwa Foods and Biosciences Inc.,
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- INAKA Koji
- Maruwa Foods and Biosciences Inc.,
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- IGARASHI Kiyohiko
- Graduate School of Agricultural and Life Sciences, The University of Tokyo VTT Technical Research Centre of Finland
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- SAMEJIMA Masahiro
- Graduate School of Agricultural and Life Sciences, The University of Tokyo
Bibliographic Information
- Other Title
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- 水素原子の可視化を目指したセルラーゼの大型結晶作製
Description
Water molecules play important roles in enzymatic reaction especially for hydrolases, which use water as a substrate. Neutron crystallography is a powerful tool for visualizing hydrogen atoms to understand protonation states of protein and orientation of water molecules, while it requires a large-volume crystal (>1 mm3 ) compared with X-ray crystallography. To overcome the hurdle of crystal volume, a counter diffusion device equipped with 2 mm diameter can be used at the microgravity experiments. We report here the crystallization of two cellulases which hydrolyse glycosidic linkages in cellulose.
Journal
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- International journal of microgravity science and application
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International journal of microgravity science and application 34 (1), 340108-, 2017-01-31
The Japan Society of Microgravity Application
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Details 詳細情報について
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- CRID
- 1390285300157612544
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- NII Article ID
- 130007842594
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- ISSN
- 21889783
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- Text Lang
- ja
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- Data Source
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- JaLC
- CiNii Articles
- KAKEN
- Crossref
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- Abstract License Flag
- Disallowed
