Structure and Biochemical Characters of Artificial Protein Cage: Assembly and Disassembly can be Controlled

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  • IWASAKI Kenji
    Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance, University of Tsukuba
  • MIYAZAKI Naoyuki
    Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance, University of Tsukuba

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Other Title
  • 開閉可能な人工タンパク質ケージの構造と性質
  • カイヘイ カノウ ナ ジンコウ タンパクシツ ケージ ノ コウゾウ ト セイシツ

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Description

<p>An ultra-stable artificial protein cage was created by using TRAP (trp RNA-binding attenuation protein). Adding a gold (I) triphenylphosphine compound to a cysteine-substituted TRAP generated a cage 22 nm in diameter. The TRAP cage resisted severe conditions, such as high temperature, denaturing agents, and acidic and basic solvents. However, it was instantly disassembled when reducing agents were added to the solution. We can therefore fully control both assembly and disassembly of the TRAP cage. Cryo-EM single-particle analysis revealed a chiral pair of TRAP cages and their atomic structures, in which gold atoms acted as staples to connect the TRAP rings.</p>

Journal

  • Seibutsu Butsuri

    Seibutsu Butsuri 60 (3), 153-156, 2020

    The Biophysical Society of Japan General Incorporated Association

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