Effect of downhill running on muscular DNA damage in rats

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  • Tadano Chigaya
    Department of Exercise Physiology, School of Medicine, Faculty of Medicine, Toho University
  • Yona Masae
    Tokyo University of Pharmacy and Life Science the School of Pharmacy
  • Naito Yuko
    Department of Sport and Medical Science, Faculty of Physical Education, Kokushikan University
  • Muro Masuo
    Department of Exercise Physiology, School of Medicine, Faculty of Medicine, Toho University

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<p>  The aim of this study was to determine oxidative stress in skeletal muscle and examine the effect of exercise on tissue oxidative stress. Male wistar rats (8 weeks old, n = 30) were used in this study. Rats performed an intermittent downhill running exercise (DH) at -17 degree incline, 25 m/min for a total of 90 min (5-min bouts separated by 2-min rest, 18 bouts). Blood lactate level slightly increased after the DH (p < 0.05). Plasma CK increased 2-3 times immediately after DH and did not return to the pre- value in 2-day recovery period (p < 0.01). Muscle glycogen decreased by 39.2% in soleus (SOL) whereas there was no difference in medial gastrocnemius white portion (GMW) and red portion (GMR) (p < 0.05). Muscular DNA damage (8-OHdG/dG) increased at 24 h after DH in both GMW and GMR, but not in SOL (p < 0.05). Lipid hydroperoxide content increased at 24 h after DH in SOL, but not in GMW and GMR (p < 0.05). SOD activity increased at 24 h after DH in all muscles. Glutathione peroxidase activity increased immediately after DH in GMW. However, glutathione reductase activity reduced at 24 h and 48 h after DH in GMW. These results indicate the effect of exercise on muscular oxidative stress suggesting involvement of massive mechanical stress in enlargement of oxidative stress in fast-twitch fibers.</p>

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