Identification of a Heterozygous Microsatellite Marker in <i>Pinus pumila</i>

DOI Web Site 1 Citations 14 References
  • Uchida Hidenobu
    Department of Biological Sciences, Kanagawa University Research Institute for Integrated Science, Kanagawa University Bunkyo Study Center, Tokyo, The Open University of Japan Depatment of Chemistry, Faculty of Science, Ochanomizu University Department of Environmental Systems Sciences, Musashino University Department of Chemical Biological Sciences, Faculty of Science, Japan Women’s University Department of Food Business, Faculty of Health and Human Life, Nagoya Bunri University
  • Kochiwa Motoki
    Department of Biological Sciences, Kanagawa University
  • Sato Takeshi
    Department of Biological Sciences, Kanagawa University Research Institute for Integrated Science, Kanagawa University
  • Inoue Kazuhito
    Department of Biological Sciences, Kanagawa University Research Institute for Integrated Science, Kanagawa University
  • Suzuki Yoshihiro
    Department of Biological Sciences, Kanagawa University
  • Maruta Emiko
    Department of Biological Sciences, Kanagawa University

Description

<p>Pinus pumila, which habits around the timber line in Japanese mountains, propagates from adventitious roots of creeping or belowground stems and from seeds that are dispersed by animals. In an attempt to detect heterozygous simple sequence repeat (SSR) alleles in the nuclear genome of P. pumila, which may be useful to identify individuals in natural populations, we performed PCR using the DNA from this species and primer sets that have been reported for P. strobus SSR (Echt et al. 1996). Aligned subclone sequences originating from RPS150 product suggested that this locus included at least two [ga(t/g)]n alleles, [gat]3[gag]3 and [gat]3[gag]1[gat]1, while the direct sequence of RPS160 product included a single SSR allele of [acag]3[gcag]1[acag]3. This is the first report of the heterozygous nuclear SSR locus in P. pumila.</p>

Journal

  • CYTOLOGIA

    CYTOLOGIA 85 (2), 131-134, 2020-06-25

    Japan Mendel Society, International Society of Cytology

Citations (1)*help

See more

References(14)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top