フォトアフィニティーラベルによる植物ペプチドホルモンの受容体探索

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  • Identification of Receptors of Plant Peptide Hormones by Photoaffinity Labeling
  • フォトアフィニティーラベル ニ ヨル ショクブツ ペプチドホルモン ノ ジュヨウタイ タンサク

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<p>In plants, there are several kinds of intercellular signal transduction systems, such as with phytohormones and with peptide hormones. Small post-translationally modified peptides, which act as cell-to-cell signaling molecules, constitute the largest group of peptide hormones with various functions in intercellular signal transductions. We explored peptide hormones by in silico gene screening coupled with biochemical peptide detection. We also identified specific direct ligand-binding receptors for these peptides. In general, receptor genes are highly redundant in Arabidopsis and thus it is often difficult to identify the specific receptors by using classical forward and reverse genetics. To overcome this problem, we identify peptide hormone receptors by detecting direct peptide hormone-receptor interaction using photoaffinity labeling with photoactivatable peptide derivatives and custom-made receptor library. This review summarizes how we discovered these peptide hormone-receptor pairs and how these pairs function in plant growth and development.</p>

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