Use of mixture of xylean Clear-Rite<sup>TM</sup>3 as an alternative reagent and alcohol for tissue delipidation with an auto tissue processor

DOI
  • YOSHIDA-TANAKA Miho
    Departments of Diagnostic Pathology, National Hospital Organization Kure Medical Center and Chugoku Cancer Center
  • KURAOKA Kazuya
    Departments of Diagnostic Pathology, National Hospital Organization Kure Medical Center and Chugoku Cancer Center Clinical Laboratory, National Hospital Organization Kure Medical Center and Chugoku Cancer Center Institute for Clinical Research, National Hospital Organization Kure Medical Center and Chugoku Cancer Center
  • KAN Arisa
    Departments of Diagnostic Pathology, National Hospital Organization Kure Medical Center and Chugoku Cancer Center
  • FUJISAWA Hiroki
    Departments of Diagnostic Pathology, National Hospital Organization Kure Medical Center and Chugoku Cancer Center
  • YASUMURA Naoko
    Departments of Diagnostic Pathology, National Hospital Organization Kure Medical Center and Chugoku Cancer Center
  • SAIKI Yumi
    Departments of Diagnostic Pathology, National Hospital Organization Kure Medical Center and Chugoku Cancer Center
  • MIYANO Hideaki
    Clinical Laboratory, National Hospital Organization Kure Medical Center and Chugoku Cancer Center
  • TANIYAMA Kiyomi
    President, National Hospital Organization Kure Medical Center and Chugoku Cancer Center

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Other Title
  • 自動包埋装置における代替キシレンクリアライト<sup>TM</sup>3とアルコール混合液を用いた脱脂の検討

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Abstract

<p><Introduction> We investigated the use of xylean Clear-RiteTM3 as an alternative reagent for tissue delipidation with an auto tissue processor. <Method> Nine breast cancer specimens totally resected from February to June 2017 were used. We prepared specimens for histopathological diagnosis (A) and specimens for routine examination (B). The A specimens were delipidated with acetone in a shaker for three hours, whereas the B specimens were delipidated with Clear-RiteTM3 and alcohol mixed with a reagent in an auto tissue processor for three hours. Subsequently, we examined the effect of delipidation on the slice graft using the automatic slice device AS-400M and the immunohistochemical staining of ER/PgR/HER2 IHC and HER2 FISH staining. <Results> The A specimens showed higher TG levels than the B specimens (p = 0.0156). In all 154 blocks, the slice success rates with the automatic slice device and the HE stainability were similar. The ER/PgR results were concordant (100%), but the HER2 IHC results in two cases were not concordant（2/9, 22.2%). The A specimens showed a false-positive result. In those two cases, there was no amplification in HER2 FISH. <Conclusion> The mixture solution of xylean Clear-RiteTM3 as the alternative reagent and alcohol had sufficient delipidation effects. The auto tissue processor enabled rapid delipidation. The risk from exposure to organic solvents was reduced.</p>

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