Validation of MALDI-TOF MS devices in reanalysis of unidentified pathogenic bacteria detected in blood cultures

  • Imai Minako
    Department of Clinical Laboratory Medicine, Fukushima Medical University Hospital
  • Kimura Yukio
    Department of Laboratory Medicine, Fukushima Medical University
  • Tanno Daiki
    Department of Clinical Laboratory Medicine, Fukushima Medical University Hospital Department of Laboratory Medicine, Fukushima Medical University
  • Saito Kyoichi
    Department of Laboratory Medicine, Fukushima Medical University
  • Honda Mutsuko
    Department of Clinical Laboratory Medicine, Fukushima Medical University Hospital
  • Takano Yukiko
    Department of Clinical Laboratory Medicine, Fukushima Medical University Hospital
  • Ohashi Kazutaka
    Department of Clinical Laboratory Medicine, Fukushima Medical University Hospital
  • Toyokawa Masahiro
    Department of Clinical Laboratory Medicine, Fukushima Medical University Hospital Department of Laboratory Medicine, Fukushima Medical University Preparing Section for New Faculty of Medical Science, Fukushima Medical University
  • Ohana Noboru
    Department of Laboratory Medicine, Fukushima Medical University
  • Yamadera Yukio
    Department of Clinical Laboratory Medicine, Fukushima Medical University Hospital
  • Shimura Hiroki
    Department of Laboratory Medicine, Fukushima Medical University

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<p>In hospital microbial laboratories, morphological and biochemical analyses are performed to identify pathogenic microbes;however, these procedures lack rapidity and accuracy. Recently, Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) has been clinically utilized, and is expected to enable rapid and accurate microbial identification. We aimed to validate two MALDI-TOF MS devices available in Japan: the VITEK-MS (BioMérieux) and the Microflex LT (Bruker Daltonics). Clinically isolated bacteria, 100 samples in all, detected in blood cultures but incompletely identified by conventional procedures, were reanalyzed using the two devices. The VITEK-MS and Microflex LT, respectively, identified 49% (49/100) and 80% (80/100) of the tested bacteria at the species level, as well as 96% (96/100) and 95% (95/100) at the genus level. Among those reidentified strains, 26% (26/100) at the species level and 88% (88/100) at the genus level were concordant with each other, though three strains were unmatched. Moreover, four bacterial strains were unable to be identified using the VITEK-MS, versus five using the Microflex LT. MALDI-TOF MS devices can provide more rapid and accurate bacterial identification than ever before;however, the characteristics of each system were slightly different;therefore, it is necessary to understand the difference in performance of MALDI-TOF MS models.</p>

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