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- Hiraishi Yumiko
- Faculty of Science and Technology, Ryukoku University
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- Ozawa Hiroyuki
- Incorporated Foundation Okinawa Prefecture Environment Science Center
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- Wakai Yoshihito
- Toba Aquarium
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- Yamanaka Hiroki
- Faculty of Science and Technology, Ryukoku University Research Center for Biodiversity Science, Ryukoku University
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- Maruyama Atsushi
- Faculty of Science and Technology, Ryukoku University
Bibliographic Information
- Other Title
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- 海棲哺乳類ジュゴンの環境DNA を定量するためのプライマーセットの開発
- カイセイホニュウルイ ジュゴン ノ カンキョウ DNA オ テイリョウ スル タメ ノ プライマーセット ノ カイハツ
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Abstract
<p>A primer set was developed for detecting environmental DNA (eDNA) of an endangered medium-sized marine mammal, Dugong dugon. We designed a species-specific primer set (target gene: 138 bp of cytochrome b region) for the dugong in silico. We examined the efficiency and specificity of the primer set using DNA derived from hair roots and faeces of the dugong, and water samples collected from an aquarium where a female dugong had been reared. Artificially synthesised DNA and water samples from an aquarium of the most closely related species (African manatee) were also used for reference. By real-time quantitative PCR using SYBR-Green I, DNA was successfully amplified from the dugong samples, whereas manatee DNA was not amplified. These results show the designed primer set to be adequately efficient and specific. These results suggest that eDNA analysis can be an effective tool in surveys of the dugong in combination with conventional methods. The range of distribution of the dugong can be re-examined using eDNA analysis to implement more effective conservation strategies.</p>
Journal
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- Japanese Journal of Conservation Ecology
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Japanese Journal of Conservation Ecology 25 (1), n/a-, 2020-02-13
The Ecological Society of Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390285300185029888
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- NII Article ID
- 130007891842
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- NII Book ID
- AA11857952
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- ISSN
- 24241431
- 13424327
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- NDL BIB ID
- 030447308
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- CiNii Articles
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- Abstract License Flag
- Allowed