Proteomic analysis of the monkey hippocampus for elucidating ischemic resistance
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- Mori Yurie
- Department of Environmental and Molecular Medicine, Mie University Graduate School of Medicine
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- Oikawa Shinji
- Department of Environmental and Molecular Medicine, Mie University Graduate School of Medicine
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- Kurimoto Shota
- Department of Environmental and Molecular Medicine, Mie University Graduate School of Medicine
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- Kitamura Yuki
- Department of Environmental and Molecular Medicine, Mie University Graduate School of Medicine College of Pharmacy, Kinjo Gakuin University
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- Tada-Oikawa Saeko
- Department of Environmental and Molecular Medicine, Mie University Graduate School of Medicine Department of Human Nutrition, School of Life Studies, Sugiyama Jogakuen University
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- Kobayashi Hatasu
- Department of Environmental and Molecular Medicine, Mie University Graduate School of Medicine
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- Yamashima Tetsumori
- Departments of Psychiatry and Neurobiology, Kanazawa University Graduate School of Medical Science
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- Murata Mariko
- Department of Environmental and Molecular Medicine, Mie University Graduate School of Medicine
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抄録
<p>It is well-known that the cornu Ammonis 1 (CA1) sector of hippocampus is vulnerable for the ischemic insult, whereas the dentate gyrus (DG) is resistant. Here, to elucidate its underlying mechanism, alternations of protein oxidation and expression of DG in the monkey hippocampus after ischemia-reperfusion by the proteomic analysis were studied by comparing CA1 data. Oxidative damage to proteins such as protein carbonylation interrupt the protein function. Carbonyl modification of molecular chaperone, heat shock 70 kDa protein 1 (Hsp70.1) was increased remarkably in CA1, but slightly in DG. In addition, expression levels of nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylase sirtuin-2 (SIRT2) was significantly increased in DG after ischemia, but decreased in CA1. Accordingly, it is likely that SIRT2 upregulation and negligible changes of carbonylation of Hsp70.1 exert its neuroprotective effect in DG. On the contrary, carbonylation level of dihydropyrimidinase related protein 2 (DRP-2) and l-lactate dehydrogenase B chain (LDHB) were slightly increased in CA1 as shown previously, but remarkably increased in DG after ischemia. It is considered that DRP-2 and LDHB are specific targets of oxidative stress by ischemia insult and high carbonylation levels of DRP-2 may play an important role in modulating ischemic neuronal death.</p>
収録刊行物
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- Journal of Clinical Biochemistry and Nutrition
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Journal of Clinical Biochemistry and Nutrition 67 (2), 167-173, 2020
一般社団法人 日本酸化ストレス学会
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詳細情報
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- CRID
- 1390285300186988160
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- NII論文ID
- 130007894919
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- ISSN
- 18805086
- 09120009
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可