Characterization of scent scales and scent scale substances of artificial hybrids between Pieris napi japonica and Pieris melete (Lepidoptera, Pieridae)

TANAHASHI Ichiro, KITAHARA Hikaru

doi:10.18984/lepid.71.3-4_75

Scent scale substances of first filial generation (F1; F1-1, F1-2) and backcrossed generation (BC; BC1 and BC2) male hybrids between Pieris napi japonica and Pieris melete were investigated using a gas chromatograph (GC) and a gas chromatograph-mass spectrometer (GC/MS). The scent scale substances of male pierid butterflies were stored in the bladder (cell) of scent scales (androconial scales) on the dorsal side of their wings. The size of the bladder of P. napi, hybrids and P. melete increased in that order. The F1 (F1-1: P. napi japonica male × P. melete female and F1-2: P. melete male × P. napi japonica female) and BC hybrids were respectively obtained by hand pairing and cage pairing. First backcrossed generation (BC1) and second backcrossed generation (BC2) hybrids were produced by mating F1-2 hybrid male × P. melete female and BC1 hybrid male × P. melete female, respectively. Main scent scale substances emitted from summer form male wings of P. napi japonica, F1-1, F1-2 and BC1 hybrids were identified as linalool and citral (a mixture of neral and geranial). On the other hand, in the case of summer form males of P. melete and BC2 hybrid, citral was the main scent scale substance. In the GC measurements, the total amount of scent scale substances (linalool plus citral) of the investigated butterflies by the solvent extraction method was larger than that by the headspace method. The ratio of the amount of linalool to citral by the solvent extraction method was smaller than that by the headspace method.

Characterization of scent scales and scent scale substances of artificial hybrids between <i>Pieris napi japonica</i> and <i>Pieris melete</i> (Lepidoptera, Pieridae)

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