Strategies for Making Multimeric and Polymeric Bifunctional Protein Conjugates and Their Applications as Bioanalytical Tools

DOI PDF Web Site Web Site Web Site ほか1件をすべて表示 一部だけ表示 参考文献65件
  • PERMANA Dani
    Department of Applied Chemistry, Graduate School of Engineering, Kyushu University Research Unit for Clean Technology, Indonesian Institute of Sciences (LIPI), Kampus LIPI Bandung
  • MINAMIHATA Kosuke
    Department of Applied Chemistry, Graduate School of Engineering, Kyushu University
  • GOTO Masahiro
    Department of Applied Chemistry, Graduate School of Engineering, Kyushu University Division of Biotechnology, Center for Future Chemistry, Kyushu University
  • KAMIYA Noriho
    Department of Applied Chemistry, Graduate School of Engineering, Kyushu University Division of Biotechnology, Center for Future Chemistry, Kyushu University

この論文をさがす

抄録

<p>Enzymes play a central role in the detection of target molecules in biotechnological fields. Most probes used in detection are bifunctional proteins comprising enzymes and binding proteins conjugated by chemical reactions. To create a highly sensitive detection probe, it is essential to increase the enzyme-to-binding protein ratio in the probe. However, if the chemical reactions required to prepare the probe are insufficiently site-specific, the detection probe may lose functionality. Genetic modifications and enzyme-mediated post-translational modifications (PTMs) can ensure the site-specific conjugation of proteins. They are therefore promising strategies for the production of detection probes with high enzyme contents, i.e., polymeric bifunctional proteins. Herein, we review recent advances in the preparation of bifunctional protein conjugates and polymeric bifunctional protein conjugates for detection. We have summarized research on genetically fused proteins and enzymatically prepared polymeric bifunctional proteins, and will discuss the potential use of protein polymers in various detection applications.</p>

収録刊行物

  • Analytical Sciences

    Analytical Sciences 37 (3), 425-437, 2021-03-10

    社団法人 日本分析化学会

参考文献 (65)*注記

もっと見る

関連プロジェクト

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ