Relationship between red blood cell aging and intracellular chemokine storage

Ogasawara Shu, Saito Norihiro, Minakawa Satoko, Akasaki Tomomi, Kayaba Hiroyuki

doi:10.32216/hirosakiigaku.71.2-4_113

Background: The Duffy antigen receptor for chemokines （DARC） is expressed on RBCs and is a non-signaling receptor for multiple chemokines. In the previous study, we had shown that DARC-affinity chemokines are stored in the RBCs. However, the fate of intracellularly stored chemokines has yet to be investigated. This study investigated whether RBCs retain the ability to accumulate these chemokines as they age.<br> Methods: Peripheral blood from five healthy volunteers were collected, and then transferred to hematocrit capillary tubes. After centrifugation, the RBC layer was divided to compare the top, upper, middle and bottom fractions. HbA1c, eotaxin-1 and RANTES were measured in each fraction.<br> Results: HbA1c increased significantly from the top fraction to the bottom fraction. Eotaxin-1 decreased significantly from the top fraction to the bottom fraction. RANTES exhibited a similar decrease, although no significant differences were observed. Eotaxin-1 showed a negative correlation（ r = -0.51, p = 0.03） with HbA1c. Although not statistically significant, RANTES showed a decreasing tendency （r = -0.40, p = 0.08） with the increase of HbA1c levels. These results indicate that RANTES and eotaxin-1 decrease rather than increase with RBC aging.<br> Conclusion: Eotaxin-1 and RANTES stored in RBCs tended to decrease as RBCs age.

Relationship between red blood cell aging and intracellular chemokine storage

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